During gastrulation, sequentially lineage-restrictive mesodermal cell populations emerge along a developmental trajectory involving primitive streak to lateral mesoderm formation, followed by cardiac ...

Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...

We characterize a population of early mesoderm-specified (EM) progenitors that is generated from mouse ESCs by bone morphogenetic protein stimulation. We further show that pluripotent ESCs are ...

hiPSC cardiac differentiation was done by modulation of the WNT/β-catenin pathway with the GSK3 inhibitor CHIR99021 to induce mesoderm differentiation and spatial organization on the PEG. Increased ...

Specifically, the endoderm tends to segregate from the neural ectoderm and preferentially localizes in non-CNS ectoderm regions, whereas the mesoderm shows a more spread localization within ...